Duration : 1 day Location : Imaging Associates, Bicester
Participant pre-requisites
Familiarity with the Windows operating system
Software Packages and options covered
AxioVision, Multi-channel, Z-Stack & Time lapse options
Objectives You will become familiar with the features of the AxioVision basic package including multi-channel fluorescence.
Topics Camera control and image capture
Image archiving and formats
Multichannel, Z-stack and time lapse image capture
Scale bars
Image annotation
Basic interactive measurements
Workflow and toolbar customisation
Importing and exporting images
Language : English
Number of participants restricted to 6
Contact : Beverley Olah (Beverley.Olah@imas.co.uk) Tel : +44 (0)1869 356240
Duration : 1 day Location : Imaging Associates, Bicester
Participant pre-requirement
Familiarity with the Windows operating system
AxioVision 4 basics
Software covered
AxioVision Automeasure module
Objectives
You will learn how to use the Automeasure option to analyse various types of images.
Topics
Understand the image processing and analysis methods used in the Automeasure wizard
Configuration of the Automeasure wizard
Using the Automeasure wizard to analyse sequences of images
Examples
Duration : 2 days Location : Imaging Associates, Bicester
Participant pre-requisites
Familiarity with the Windows operating system
Familiarity with AxioVision basics
Software Packages and options covered
AxioVision, Imaging Plus, Automeasure, Automeasure Plus & Commander options
Objectives
You will learn how to use image processing functions and measurement functions to analyse an image. You will be taught how to use 'Commander' to generate scripts for automation.
Topics
Strategies for image analysis
Explanation and application of image processing functions (contrast operations, filtering operations, morphological operators)
Segmentation functions for object recognition
Explanation and application of binary operators
Measurement parameters, calibration and automatic image measurement
Advanced interactive image measurement
Creating Commander scripts
Running Commander scripts in batch mode
Language : English
Number of participants restricted to 6
Contact : Beverley Olah (Beverley.Olah@imas.co.uk) Tel : +44 (0)1869 356240
Note : The AxioVision ‘Mark and Find’ module is not covered in this course
Duration : 1 day Location : Imaging Associates, Bicester
Participant pre-requisites
Familiarity with the Windows operating system
Familiarity with AxioVision basics
Some experience of fluorescence microscopy
Software Packages and options covered
AxioVision, Apotome, 3D Deconvolution, Inside 4D
Objectives
To understand how optical sections can be obtained from thick specimens. You will learn how to acquire stacks of optical sections and how to present 3D information.
Topics
Multichannel and Z-stack image capture
Background to the Apotome technique
Calibration of an Apotome
Use of an Apotome
Background to deconvolution theory
Deconvolution pre-requisites
Deconvolving images - comparison of methods
3D reconstruction techniques
Creating and exporting 3D animations
Language : English
Number of participants restricted to 6
Contact : Beverley Olah (Beverley.Olah@imas.co.uk) Tel : +44 (0)1869 356240
